Tutorials
In effort to provide the tools required to support quality testing for onchocerciasis, tutorials will be provided below as they become available.
Preparing Black Fly Pool Homogenate / Supernatant
The following video describes how to sort black fly pools and extract the homogenate or supernatant that will be used in downstream DNA Extraction for use in the O150 PCR/ELISA
Black Fly Pool Screen Programs Available:
(For post-elimination evaluation using black fly surveillance and O150 PCR results)
PoolTools
Tutorial on using PoolTools to estimate prevalence is available at the following link:
PoolTestR
Pool Screen 2.0 & Pool Screen OEPA
Available upon request (Shipped on a USB)
The following video describes how to utilize the black fly pool screening programs to evaluate prevalence of infective larvae in black fly collections to aid in determination of onchocerciasis elimination / post-elimination surveillance.
Pool Screen Programs: "Pool Screen 2.0" and "Make Hour Calculator" combined with the "Pool Screen OEPA".
Future training tutorials will be added as they become available.
If you would like to see a specific training application for the Ov16 ELISA or O150 PCR/ELISA, please send us your request at ovlabnetwork@gmail.com